Aflatoxin M1, is a toxic metabolite of aflatoxin B1. It is normally excreted in the urine and also the milk of dairy cattle and other lactating mammals. Due to the high toxicity of aflatoxin M1, the European Community established the maximum level of 0.05µg/kg for aflatoxin M1 in liquid milk. In case of infant and follow-on milk the limit is 0.025µg/kg. This limits is also applicable to milk products which are dried or processed, taking into account the concentration caused by the drying process or by processing. By the other side, the Codex Alimentarius and FDA has established an acceptable level of risk at 0. 5µg/kg for aflatoxin M1 in fluid milk.
Principle of the analysis: Competitive enzyme immunoassay